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KMID : 0854720000200060943
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Korean Journal of Asthma, Allergy and Clinical Immunology
2000 Volume.20 No. 6 p.943 ~ p.959
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The Inhibitory Mechanism of Alprogen II α on the Mediator Release in the Mast Cells Sensitized with House Dust Mite Antigen
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Jai-Youl Ro/Young-In Park1
Ji-Young Kim2/Yean-Jun Chung1/Jai-Youl Ro2
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Abstract
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Background : We reported that the single glycoprotein alprogen extracted from Aloe strongly inhibited the mediator releases caused by the activation of guinea pig lung mast cells. We purified another compound alprogen II α from Aloe vera. Therefore, this study aimed to assess the effects of Aloe single component (alprogen II α) on the mechanism of mediator releases caused by the mast cell activation,
Materials and methods : We purified Aloe extracts by using various columns. #We also purified mast cells from guinea pig lung tissues by using enzyme digestion, the rough and discontinuous density percoll gradient method. Mast cells were sensitized with IgG$gt; (anti-Ox-HDM) and challenged with Ox-HDM. Histamine was assayed by using fluorometric analyzer. leukotrienes by radioimmunoassay. Intracellular Ca++ level was analyzed by using a confocal laser scanning microscope. Protein kinase C (PKC) activity was determined by the protein phosphorylated with [γ-(32)^P]ATP. The phospholipase D (PLD) activity was assessed bye labeled phosphatidylalcohol. PLA₂ activity was determined by measuring the lyso-phosphatidylcholine released from the labeled phospholipids.
Results: Alprogen IIα significantly decreased histamine, leukotriene, and TNF-α releases, and blocked completely Ca++ influx during mast cell activation. The PLD and PKC activities were dec reased in a dose-dependent manner. Alprogen IIα inhibited the PLA2 activity during mast cell activation.
Conclusion: The data suggest that alprogen IIα purified from Aloe vera inhibit mediator release by blocking the initial signal cascade of activation of mast cell stimulated with Ox-HDM/anti-Ox-HDM antibody reaction.
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